Dako Hybridizer User Manual download pdf (Page 4)

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Run a Denaturation and Hybridization Program (Denat & Hyb)

1. Select Run a PGM 2. Select a PGM

n Cursor highlights “Run a

PGM” line

n Press “Enter” button to accept

n Scroll through program

Nos. 1-40/program names

n Press “Enter” button to accept

n Display will conrm PGM No./

name and Denat & Hyb times

and temperatures

n Cursor highlights “Run PGM”

line

n Press “Enter” button to accept

3. Insert Humidity Control Strips and Add Slides 4. Heating to Denat Temp

n Insert two Humidity Control

Strips into the inside slide lid

n Saturate strips with distilled

water or equivalent

n Add slides and close lid

n Cursor highlights “Start” line

n Press “Enter” button to run the

program

n Display indicates present

temperature of the slides

5. Denaturation 6. Cooling to Hyb Temp

n The Hybridizer will beep twice

once temperature reaches

denaturation set point

n Denaturation time will count

down from the set time

n The Hybridizer will

automatically cool to

hybridization set temperature

once denaturation is

completed

7. Hybridization 8. Process Completed

n Hybridization time will count

down from the set time

once temperature reaches

hybridization set point

n The Hybridizer will beep

ve times upon program

completion

n Cursor highlights “End PGM/

Main Menu”

n Press “Enter” to end program

n See Handbook for further

details and for running a

“Hyb Only” or “Fixed Temp”

program

Run a PGM

Edit a PGM

Create a PGM

Present Temp: 37 °C

PGM 01 HER2

82 °C :05; 45 °C 20:00

Run PGM

Main Menu

PGM 01 HER2

Add Slides-Close Lid

Star t

Main Menu

PGM 01 HER2

**Heating**

Denat: 82 °C :05

Present Temp: 45 °C

PGM 01 HER2

Denat in Process

Denat: 82 °C 02:28

Present Temp: 82 °C

Please Wait

Cooling to Hyb 45 °C

Present Temp: 58 °C

PGM 01 HER2

Hyb in Process

Hyb: 45 °C 12:48

Present Temp: 45°C

PGM 01 HER2

PROCESS COMPLETE

Total Hyb Time 20:00

End PGM/Main Menu

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Dako Hybridizer User Manual Page 4

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