Dako Hybridizer User Manual download pdf (Page 26)

26 Dako Hybridizer | Handbook

Recommended for Best Results

 Place glass coverslip over probe and sample

 Apply sealant along all coverslip edges

 Use Dako Humidity Control Strips S 2452

Slide Location

Hybridizer accommodates up to 12 slides per run. When prompted, lift lid and load slides onto plate using

slide locator for easy positioning. Frosted and/or label end of slides should be away from slide locator.

Move slides across plate until they rest against slide locator.

Note: If slides are not resting against slide locator, closing of lid may break slides.

Humidity Control Strips

Humidity Control Strips are used to minimize evaporation of assay solution. Located in the lid these strips

act to prevent evaporation of probe solution from prepared slides.

Instructions for use: After locating the slides, saturate strips with distilled water or deionized water

(approx. 13 mL for unused strips).

Instructions for reuse: After the run has ended, keep lid closed between runs to avoid drying out of the

strips. Do not reuse strips that have been dried out after initial saturation. Resaturate the strips before

starting a new run. The amount of water needed to resaturate the strip depends on the ISH procedure

and the time in between runs. Strips should be replaced frequently as they will degrade over time and

with use. See package insert for Hybridizer Humidity Control Strips for further instructions. Replacement

strips can be purchased for use with the Hybridizer.

If the Dako Hybridizer instrument has to stand unused for more than four days, open the lid, remove strips

and allow instrument to dry. It is recommended to allow the instrument to dry as described above with

each strip change and that the users keep the inner surface of the instrument in a clean condition. For

cleaning instructions advance to the cleaning section of this handbook.

To replace strips, lift lid and remove strips. Insert strips into slot positions; allow fingers in lid to support

strips.

Note: Do not use paper towels in strip positions, they must be folded and damage may result in lid

fingers.

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Dako Hybridizer User Manual Page 26