Dako Autostainer User Manual download pdf (Page 27)

Section 6

Programming a Staining Run

The Autostainer can process a maximum of 48 microscope slides in a single staining

run. Each slide can be stained using an independently designed protocol. After the slide

information is entered and protocol template is selected, specific reagents are assigned

to each slide. This is accomplished from the Programming Grid. The following section

describes how to program specific reagents for a staining run.

Each row

represents one

slide and its

information.

Reagents that

are available for

the highlighted

step.

Defining Detection Reagents

Before you can program specific reagents for slides, you must enter slide information

and select a protocol template as described in Section 4 and Section 5. The

Programming Grid is displayed listing the slides identified during Slide Count or Slide

Information entry. The first programmable field is highlighted and a list of available

reagents for the step is displayed.

1. Select a reagent using the UP and DOWN arrow keys and insert the reagent by

pressing ENTER. A message asks, “Assign to the following unprogrammed slides?”

2. Select Yes to assign the selected reagent to the remaining slides in the grid and the

next programmable step is highlighted. Select No and the reagent is assigned to only

the highlighted step.

3. After all reagents are programmed, click the Next button. The system checks the grid

for missing rinses. If a rinse is missing between two reagents, a message asks if you

want to continue.

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Dako Autostainer User Manual Page 27