Dako Autostainer User Manual download pdf (Page 36)

Adding Primary Antibodies

A primary antibody can be added to the reagent list with a related pretreatment reagent.

This facilitates automatic selection of the pretreatment whenever a primary antibody is

selected from the Programming Grid.

1. Follow the same steps as in the Adding Detection Reagents section.

Note: To add a matching negative control use exactly the same name as the positive

control and add the prefix “_NC” to the beginning of the reagent name.

2. If the primary antibody is a step that is preformed off the Autostainer (i.e., overnight

incubation) or requires heating, check the Manual Step check box by pressing the

SPACE BAR.

3. Enter the correct compatibility code and press ENTER. Assigning compatibility

information to reagents is optional (see Compatibility Check at the end of this

section).

4. Select the pretreatment from the box by using the UP and DOWN arrows, or type a

new pretreatment name, and press ENTER

Note: If Manual Step is selected, the incubation time will be “0”. Do not change this

time.

Editing and Deleting Reagent Lists

1. From the Programming Grid select Edit Lists on the menu bar.

2. Select the reagent category from the list.

3. Edit fields by highlighting the information and typing over it. When changing the

reagent name, the software saves the change as a new reagent.

4. To delete, click the Delete button.

5. Select the OK button.

Updating a Reagent Lot Number and Expiration Date

1. From the Programming Grid select Edit Lists on the menu bar.

2. Select the reagent category from the list, and choose a specific reagent.

3. Highlight the current lot number displayed in the lot box. Type the new lot number

and the Change button appears.

4. Highlight the date displayed in the box and enter the new expiration date.

5. Click the Change button – all fields clear.

6. Select the OK button until a dialog box prompts “Save changes on disk?”

7. Select Yes to save the new lot number and expiration date. Select the No button and

the reagent is not edited.

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Dako Autostainer User Manual Page 36