Dako Autostainer User Manual Page 7

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Table of Contents
Warnings, Precautions and Limitations............................................................................. 1
System Overview .............................................................................................................. 5
Software Overview ............................................................................................................ 7
Sign In Screen............................................................................................................... 7
Main Menu .................................................................................................................... 7
Initialize .........................................................................................................................8
Label Printing Initialization ........................................................................................8
Communication Port..................................................................................................9
Printer Selection......................................................................................................10
The Options Screen ................................................................................................ 10
Format Program Grid .............................................................................................. 10
IHC Report .............................................................................................................. 10
Custom Options ......................................................................................................10
Reagent Volume and Drop Zone Default ................................................................11
Clean........................................................................................................................... 11
Sign Off and Help........................................................................................................ 11
Reagent Tracking........................................................................................................ 11
Prime Pump ................................................................................................................12
Programming Grid Overview....................................................................................... 12
Entering Slide Information............................................................................................... 13
Adding Slides .............................................................................................................. 13
The Slide Info Button................................................................................................... 13
Deleting Slides ............................................................................................................ 14
Deleting Slides With the Slides Function.................................................................14
Deleting Slide ID Information Using the Slide Info Button.......................................14
Rearranging slide positions on the Programming Grid ...............................................14
Designing a Protocol.......................................................................................................15
Standard Protocol Elements ....................................................................................... 15
Additional Protocol Elements ...................................................................................... 16
Rinse Buffer ............................................................................................................16
Rinse Water ............................................................................................................16
Standard Rinse Replacement ................................................................................. 16
Substrate-Batch ......................................................................................................16
Switch......................................................................................................................16
Creating and Editing a Protocol Template .................................................................. 16
Deleting a Protocol Template...................................................................................... 17
Using a New Protocol Template Without Saving ........................................................17
Using a Saved Protocol Template...............................................................................17
Using a Saved Protocol Template for a Specific Auto Program..................................18
Selecting Reagent Dispense Volume for All Steps in a Protocol ................................ 18
Selecting Reagent Dispense Volume for a Specific Step in a Protocol ...................... 18
Programming a Staining Run .......................................................................................... 19
Defining Detection Reagents ......................................................................................19
Defining Primary Antibodies........................................................................................ 20
Assigning Positive and Negative Control Reagents.................................................... 20
Adding Positive and Negative Control Reagents .................................................... 21
Autostainer Handbook
vii
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