Dako Autostainer User Manual download pdf (Page 104)

104 Dako Autostainer | User Guide

Reagent Preparation

One Liter of Tris-Buffered Saline Solution (S3001)

1. Add 1L (1000 mL) of deionized water to a 1L carboy, flask or beaker.

2. Empty entire contents of one packet. Use a magnetic stir plate to mix and dissolve the

TBS granules.

3. Add 0.5 mL of Dako Tween 20 (Code No. S1966) to the Tris-buffered saline solution and

mix using a magnetic stir plate.

4. The final solution is 50mM Tris-HCl, 150mM NaCl buffered saline, pH 7.6 with 0.05%

Tween 20.

Five Liters of Tris-Buffered Saline Solution (S1968)

1. Add 5 L (5000 mL) of deionized water to a 5 L carboy, flask or beaker.

2. Empty entire contents of one packet. Use a magnetic stir plate to mix and dissolve the

TBS granules.

3. Add 2.5 mL of Dako Tween 20 (Code No. S1966) to the Tris-buffered saline solution and

mix using a magnetic stir plate.

4. The final solution is 50mM Tris-HCl, 150mM NaCl buffered saline, pH 7.6 with 0.05%

Tween 20.

Ten Liters of Tris-Buffered Saline Solution (S3006)

1. Prior to dilution, allow the Wash Buffer 10X solution to reach room temperature (20-

25C).

2. Add 1L (1000mL) of concentrated Wash Buffer 10X solution to a 10L carboy, flask or

beaker.

3. Add 9L (9000mL) of deionized water to the same container. Use a magnetic stir plate to

stir gently for at least 15 minutes.

4. The final solution is 50mM Tris-HCl, 150mM NaCl buffered saline, pH 7.6 with 0.05%

Tween and a preservative.

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Dako Autostainer User Manual Page 104