Dako Autostainer User Manual download pdf (Page 107)

Dako Autostainer | User Guide 107

Dako Phosphate Buffered Saline

Dako Phosphate Buffered Saline Solution can be used for Autostainer reagent preparation and

general laboratory use.

Intended Use

Dako recommends phosphate buffered saline (PBS) in the preparation of many immunohisto-

chemical and in situ hybridization procedures.

Materials Required

1. Clean carboy, flask or beaker graduated to 1 Liter

2. Deionized water

3. Magnetic stir plate with stir bar

4. Prepackaged Dako reagents

Code No. Contents

S3024 Six packets of PBS containing sodium phosphate dibasic, sodium phosphate

monobasic, and sodium chloride. Each packet makes 1 liter of 20mM sodium

phosphate, 150mM NaCl, pH 7.0.

Reagent Preparation

One Liter of Phosphate Buffered Saline Solution (S3024)

1. Add l L (1000 mL) deionized water a 1L carboy, flask or beaker.

2. Empty entire contents of one packet. Use a magnetic stir plate to mix and dissolve the

PBS granules.

3. Add 0.5 mL Dako Tween 20 (Code No. 1966) to the phosphate buffered saline solution

and mix using a magnetic stir plate.

4. The final solution is 20mM sodium phosphate, 150mM NaCl, phosphate buffered saline,

pH 7.0 with 0.05% Tween 20.

Shelf Life and Storage

PBS solutions should be stored at 2-8° C to inhibit bacterial growth. PBS solutions which becomes

turbid should be discarded.

If phosphate buffered saline is stored at 4° C, the expected shelf life is 7 days.

If phosphate buffered saline is stored at room temperature, the expected shelf life is 4 days.

NOTE: Dako PBS does not contain any preservatives. It is recommended that the wash buffer

carboy is washed according to good laboratory practices each time before it is filled with fresh

buffer. Also, it is recommended that the end piece of the in-line buffer tubing is wiped clean

periodically to avoid clogging.

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Dako Autostainer User Manual Page 107