Dako Autostainer User Manual Page 80

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80 Dako Autostainer | User Guide
If more than 32 reagent vials are required by the run, a SECOND RACK button becomes available at
the bottom of the screen.
The buttons and their functions are:
REAGENT LIST
displays a detailed listing of the reagents used in the current staining
run.
NEXT
displays the SLIDE LAYOUT MAP:LOAD SLIDES screen.
BACK TO GRID
returns to the Programming Grid.
SLIDE MAP
displays the SLIDE LAYOUT MAP:Review Slides screen.
FIRST/ SECOND
RACK
displays the alternate reagent rack when more than 32 reagents are
used in a run. If more than 32 reagents are required for a run, this
button is labeled SECOND RACK and a second rack can be accessed
from this button. The background for the second rack is blue and the
vial positions appear with a “b” prefix (for example, A2b indicates
position A2 in the second/back rack). When the second rack is not
available, this button is labeled FIRST Rack and is grayed out and
inactive.
PRINT
prints the reagent layout map.
1. The reagent volume displayed in the REAGENT LAYOUT MAP screen includes a dead
volume of at least 200 µL.
2. Load the reagent vials according to the reagent map.
NOTE: Using the Reagent Layout map information, make sure that enough reagent is in
the vials to complete the programmed run and that the reagent rack is properly seated in
its correct location and orientation.
NOTE: Check that the reagent vials have no bubbles on top of the solution. Remove any
bubbles.
Reagent List Screen
To view a detailed listing of the reagents used in the current run, click on the REAGENT LIST button
in the REAGENT LAYOUT MAP screen.
Select the PRINT button to generate a detailed reagent list.
Select the OK button to return to the REAGENT LAYOUT MAP screen.
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